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On 18 April 2020, Ho was arrested as one of 15 Hong Kong high-profile democracy figures, on suspicion of organizing, publicizing or taking part in several unauthorized assemblies between August and October 2019 in the course of the anti-extradition bill protests. Following protocol, the police statement did not disclose the names of the accused, however others arrested include veteran lawyers Martin Lee and Margaret Ng.

She lost in the 2016 Hong Kong legislative election with 19,376 votes, placed ninth in the Hong Kong Island constituency, along with the former party chairman Lee Cheuk-yan, leaving the Labour Party only one representative in the legislature.

In 2012, soon after Ho re-elected as member of Legco, she put forward a proposal calling for public consultation on legislation to outlaw discrimination based on sexual orientation. This proposal was supported by most of the pro-democrats and some pro-Beijing lawmakers, but was veto by Democratic Party lawmaker James To (who voted abstained) and pro-Beijing conservative parties. During her terms, Ho is one of only few legislators to openly support same-sex marriage and other LGBT rights, conservative parties and groups criticized her on this issue. Though most of the pro-democrats support non-discrimination laws, they are less openly discuss and talk about this issue, as the pro-democrats have many supporters with many social conservative views.

She is a founding member of the Labour Party, since December 2011, and currently holds the position of vice-chairwoman. Previously, she was a founding member of The Frontier, another pro-democracy political group. Since 2006, she has been a founding councillor of the World Future Council.

In December 2011, after The Frontier had failed to pass its own resolution to dissolve, she joined with Lee Cheuk-yan, Cheung Kwok-che and others to form the Labour Party, taking up the position of vice-chairwoman. She was re-elected in 2012 elections.

Ho stood for and was returned in the Hong Kong Island geographical constituency for the 2008 Hong Kong legislative election. She finished with the fifth highest number of votes in the six-member constituency, 30,887, or 9.85%. Her running mate in 2004, Audrey Eu of the Civic Party, obtained 30,362.

In 2007, when the death of Ma Lik vacated a Hong Kong Island Legislative Council seat, she originally decided to run in the by-election, but later she decided against. Also, she declined to stand again for her district council seat to prepare for her 2008 Legco election campaign.

In addition to remaining as a key member of the Frontier until 2006, Ho hosted weekly radio programmes on two local radio stations in 2006 – on RTHK and PRHK. She hosted a radio show on the internet radio station My Radio on Thursday nights (2200–2300 HKT).

In 2006, she quit the Frontier and became the chairperson of the Hong Kong Human Rights Monitor and the co-convener of Project Civil Referendum, a scheme to pilot and promote the idea of holding a referendum in Hong Kong.

In 2004, she, together with James To Kun Sun, Frederick Fung Kin Kee, Wong Sing Chi, Mak Kwok Fung, attempted to cross the border and meet Beijing officials at Shenzhen, to request a faster pace of democratisation in Hong Kong.

The 2004 Legislative Council election returned candidates to office based on a party-list proportional representation system. There were four viable tickets running for election for the Hong Kong Island constituency. The left was represented by the Democratic Alliance for the Betterment of Hong Kong's ticket, consisting of Ma Lik and Choy So-yuk; and the centrist ticket was represented by Rita Fan.

She was elected as a District Council member of the Central and Western district via the Kwun Lung (觀龍) constituency in November 2003, unseating veteran Ip Kwok-him of the DAB with a margin of 64 votes. She also supported members of other pro-democracy political groups such as Civic Act-up, which were also contesting seats in the Wanchai district council.

She was elected a legislative councillor for the geographical constituency of New Territories East in 1998 in the first Legco election since the transfer of sovereignty over Hong Kong from the UK to China. She was re-elected in the c in 2000. She chaired bills committees such as the 2004 Education (Revised) Bill and the 2004 Examination Authority Bill, and was vice-chair of the panel on environmental affairs. She chaired the Sub-committee under the Home Affair Panel to study questions of discrimination based on sexual orientation between 2000 and 2004.

In 1991, Ho helped Emily Lau during the election campaign, the first open direct election of Legco in Hong Kong. In 1993, she founded the liberal pressure group "United Ants" with other pro-democracy political activists. In 1995, she worked as an assistant to Margaret Ng, a lawmaker representing the legal profession in Legco. In 1996, along with other political activists, she founded The Frontier.

Ho studied at the University of Waterloo, Canada. She worked in the textile trading industry from 1979 to 1995.