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Since 2017, she has starred as Serena Joy Waterford in the acclaimed Hulu drama series The Handmaid's Tale. For her performance, Strahovski earned a Primetime Emmy Award nomination for Outstanding Supporting Actress in a Drama Series in 2018.

In 2014, Strahovski joined Fox's TV series 24: Live Another Day cast as Kate Morgan, CIA agent. Later that year, she was cast as Rene Carpenter on the ABC limited series The Astronaut Wives Club. In 2016, she starred, along with Adrien Brody, as Caroline Crowley in the film noir Manhattan Night. She was featured in Maxim Hot 100 from 2009 to 2013.

In December 2012, she made her Broadway debut in a revival of Clifford Odets' Golden Boy, for which she won a Theatre World Award. Strahovski was honored along with Liam Hemsworth for their work in international roles with the 2012 Australians in Film Breakthrough Award.

Strahovski voices Aya Brea in the English version of the Parasite Eve spinoff, The 3rd Birthday, which was released on March 2011 for the PlayStation Portable. She also appeared in a CollegeHumor sketch in April 2011, parodying the music styles of Katy Perry, Ke$ha, Lady Gaga, and Justin Bieber.

Strahovski's other notable works include Lego: The Adventures of Clutch Powers (2010), Killer Elite (2011), I, Frankenstein (2014), The Astronaut Wives Club (2015), Manhattan Night (2016), He's Out There (2018), and The Predator (2018). She also voiced Miranda Lawson in the Mass Effect video game series and Batwoman in the animated superhero film Batman: Bad Blood (2016).

Strahovski sent in her audition tape for the TV series Chuck while in the United States auditioning for roles in other shows, namely NBC's 2007 series Bionic Woman. She was contacted by the producers of Chuck the next day to come in and run lines with Zachary Levi. The producers called her back a week later letting her know she had been cast as Sarah Walker. Six months later, she moved to the United States. Strahovski adopted the more phonetic spelling of Strahovski as her stage name in place of Strzechowski at this time, at producer Josh Schwartz's behest for the sake of easier pronunciation.

Strahovski was born in Werrington Downs, New South Wales, Australia the daughter of Piotr and Bożena Strzechowski; her parents emigrated from Warsaw, Poland. Her father is an electronic engineer, and her mother is a lab technician. Strahovski began acting lessons at age 12 and spent her high school years attending Santa Sabina College, Strathfield. She attended the University of Western Sydney's Theatre Nepean in 2003 and co-founded a small theater company.

Yvonne Jaqueline Strzechowski (born 30 July 1982), known professionally as Yvonne Strahovski (/s t r ə ˈ h ɒ v s k i / ), is an Australian actress. She is best known for her roles as CIA Agent Sarah Walker in the NBC spy drama series Chuck (2007–2012), Hannah McKay in the Showtime drama series Dexter (2012–2013), and CIA Agent Kate Morgan in the Fox event series 24: Live Another Day (2014). She stars as Serena Joy Waterford in the Hulu drama series The Handmaid's Tale (2017–present), for which she has received a Primetime Emmy Award nomination.